Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
5000 pg/ml | 2.258 | 2.332 | 2.219 |
2.405 | |||
2500 pg/ml | 1.281 | 1.239 | 1.126 |
1.196 | |||
1250 pg/ml | 0.707 | 0.678 | 0.565 |
0.648 | |||
625 pg/ml | 0.4 | 0.408 | 0.295 |
0.415 | |||
312.5 pg/ml | 0.274 | 0.259 | 0.146 |
0.243 | |||
156.25 pg/ml | 0.193 | 0.189 | 0.076 |
0.184 | |||
78.125 pg/ml | 0.147 | 0.151 | 0.038 |
0.154 | |||
0 pg/ml | 0.119 | 0.113 | 0 |
0.106 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 307 | 15.2 | 5 |
Intra-assay | 2 | 20 | 652 | 31.7 | 4.9 |
Intra-assay | 3 | 20 | 2609 | 129 | 5 |
Inter-assay | 1 | 20 | 308 | 25.8 | 8.4 |
Inter-assay | 2 | 20 | 649 | 46 | 7.1 |
Inter-assay | 3 | 20 | 2597 | 148 | 5.7 |
Protocols
References
Customer reviews and Q&As