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Home > Product > ELISA Kit > Rat Hemoglobin Subunit Beta-1, HBB ELISA Kit

Rat Hemoglobin Subunit Beta-1, HBB ELISA Kit

Rat Hemoglobin Subunit Beta-1, HBB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.52ng/L

Detection range: 5-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 2.52ng/L

Detection range: 5-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Hemoglobin subunit beta-1

Code

E0861Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.52ng/L

Detection range

5-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Hemoglobin subunit beta, (beta globin, β-globin, haemoglobin beta, hemoglobin beta) is a globin protein, coded for by the HBB gene, which along with alpha globin (HBA), makes up the most common form of haemoglobin in adult humans, hemoglobin A (HbA). It is 147 amino acids long and has a molecular weight of 15,867 Da. Normal adult human HbA is a heterotetramer consisting of two alpha chains and two beta chains. HBB is encoded by the HBB gene on human chromosome 11. Mutations in the gene produce several variants of the proteins which are implicated with genetic disorders such as sickle-cell disease and beta thalassemia, as well as beneficial traits such as genetic resistance to malaria.

UniProt accession

MASS(Da)

15,979

GeneID

24440

Synonyms

Beta-1-globin;HBB;Hemoglobin beta chain, major-form;Hemoglobin beta-1 chain;Hemoglobin subunit beta-1

Gene names

Hbb

Research area

Others

Target protein

HBB

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0861Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.156 2.093 2.038
2.03
600ng/L 1.68 1.591 1.536
1.501
300ng/L 0.998 1.026 0.971
1.053
150ng/L 0.71 0.732 0.677
0.753
75ng/L 0.468 0.466 0.411
0.464
0ng/L 0.051 0.054 0
0.058

Precision

We measured random samples of E0861Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 242.05 19.39 8.01
Intra-Assay 2 18 515.85 24.4 4.73
Intra-Assay 3 18 101.68 6.66 6.55

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0861Ra

    $320.00

  • 96T

    E0861Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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