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Home > Product > ELISA Kit > Rat Cytochrome P450 19A1, CYP19A1 ELISA Kit

Rat Cytochrome P450 19A1, CYP19A1 ELISA Kit

Rat Cytochrome P450 19A1, CYP19A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.03ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.03ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Cytochrome P450 19A1

Code

E0912Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.03ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Aromatase, also called estrogen synthetase or estrogen synthase, is an enzyme responsible for a key step in the biosynthesis of estrogens. It is CYP19A1, a member of the cytochrome P450 superfamily (EC 1.14.14.1), which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The enzyme aromatase can be found in many tissues including gonads (granulosa cells), brain, adipose tissue, placenta, blood vessels, skin, and bone, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer.[citation needed] It is an important factor in sexual development.

UniProt accession

MASS(Da)

58,412

GeneID

25147

Synonyms

Aromatase;Cyp19;Cyp19a;CYP19A1;CYPXIX;Cytochrome P450 19A1;Cytochrome P-450AROM;Estrogen Synthase;p450arom;

Gene names

Cyp19a1

Research area

Others

Target protein

CYP19A1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0912Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 1.967 1.975 1.946
1.983
8ng/ml 1.525 1.447 1.418
1.37
4ng/ml 0.58 0.604 0.574
0.628
2ng/ml 0.394 0.418 0.389
0.442
1ng/ml 0.173 0.172 0.142
0.17
0ng/ml 0.027 0.029 0
0.032

Precision

We measured random samples of E0912Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.7 0.22 2.88
Intra-Assay 2 18 6.32 0.36 5.66
Intra-Assay 3 18 2.61 0.1 4

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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