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Home > Product > ELISA Kit > Mouse DNA Damage-inducible Transcript 3 Protein, DDIT3 ELISA Kit

Mouse DNA Damage-inducible Transcript 3 Protein, DDIT3 ELISA Kit

Mouse DNA Damage-inducible Transcript 3 Protein, DDIT3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 0.09ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

DNA damage-inducible transcript 3 protein

Code

E1596Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.09ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotic transcription factor that is encoded by the DDIT3 gene. It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, and has an important role in the cell's stress response.

UniProt accession

MASS(Da)

19,189

GeneID

13198

Synonyms

AltDDIT3;C/EBP zeta;C/EBP-homologous protein;C/EBP-homologous protein 10;CCAAT/enhancer-binding protein homologous protein;ch;chop;CHOP-;CHOP10;CHOP-10;Ddit3;DDIT-3;DNA damage-inducible transcript 3 protein;gadd15;Growth arrest and DNA-damage-inducible protein GADD153

Gene names

Ddit3

Research area

Epigenetics and Nuclear Signaling

Target protein

Ddit3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Mouse DNA Damage-inducible Transcript 3 Protein, DDIT3 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 1.864 1.938 1.894
2.012
16ng/ml 0.957 0.953 0.909
0.949
8ng/ml 0.436 0.444 0.4
0.452
4ng/ml 0.301 0.252 0.208
0.203
2ng/ml 0.169 0.121 0.077
0.074
0ng/ml 0.055 0.044 0
0.033

Precision

We measured random samples of E1596Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.45 0.73 4.75
Intra-Assay 2 18 28.43 0.74 2.62
Intra-Assay 3 18 7.08 0.33 4.59

Protocols

References

Customer reviews and Q&As

No reviews
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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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