Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1618Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 1.82 | 1.898 | 1.852 |
| 1.976 | |||
| 600ng/L | 1.096 | 1.011 | 0.964 |
| 0.926 | |||
| 300ng/L | 0.593 | 0.585 | 0.539 |
| 0.578 | |||
| 150ng/L | 0.215 | 0.246 | 0.199 |
| 0.276 | |||
| 75ng/L | 0.078 | 0.094 | 0.048 |
| 0.111 | |||
| 0ng/L | 0.041 | 0.046 | 0 |
| 0.052 |
Precision
We measured random samples of E1618Ra within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1087.1 | 38.53 | 3.54 |
| Intra-Assay | 2 | 18 | 679.5 | 39.51 | 5.81 |
| Intra-Assay | 3 | 18 | 334.94 | 17.13 | 5.11 |
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