Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1749Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 2.123 | 2.128 | 2.078 |
| 2.133 | |||
| 4ng/ml | 1.384 | 1.428 | 1.378 |
| 1.471 | |||
| 2ng/ml | 0.772 | 0.802 | 0.752 |
| 0.832 | |||
| 1ng/ml | 0.466 | 0.481 | 0.432 |
| 0.497 | |||
| 0.5ng/ml | 0.228 | 0.262 | 0.212 |
| 0.296 | |||
| 0ng/ml | 0.063 | 0.05 | 0 |
| 0.036 |
Precision
We measured random samples of E1749Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.41 | 0.09 | 6.74 |
| Intra-Assay | 2 | 18 | 4.39 | 0.17 | 3.82 |
| Intra-Assay | 3 | 18 | 2.72 | 0.18 | 6.51 |
Protocols
References
Customer reviews and Q&As
