Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E1785Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.671 | 2.554 | 2.495 |
| 2.438 | |||
| 600ng/L | 1.33 | 1.374 | 1.316 |
| 1.419 | |||
| 300ng/L | 0.874 | 0.9 | 0.841 |
| 0.925 | |||
| 150ng/L | 0.472 | 0.429 | 0.37 |
| 0.386 | |||
| 75ng/L | 0.266 | 0.237 | 0.178 |
| 0.208 | |||
| 0ng/L | 0.055 | 0.059 | 0 |
| 0.063 |
Precision
We measured random samples of E1785Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 138.88 | 8.79 | 6.33 |
| Intra-Assay | 2 | 18 | 337.56 | 19.18 | 5.68 |
| Intra-Assay | 3 | 18 | 771.15 | 26.69 | 3.46 |
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