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Home > Product > ELISA Kit > Rat Cholesterol Side-chain Cleavage Enzyme, Mitochondrial, CYP11A1 ELISA Kit

Rat Cholesterol Side-chain Cleavage Enzyme, Mitochondrial, CYP11A1 ELISA Kit

Rat Cholesterol Side-chain Cleavage Enzyme, Mitochondrial, CYP11A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.24ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 5.24ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Cholesterol side-chain cleavage enzyme, mitochondrial

Code

E1907Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.24ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).

UniProt accession

MASS(Da)

60,586

GeneID

29680

Synonyms

Cholesterol desmolase;Cholesterol side-chain cleavage enzyme, mitochondrial;Cyp11a;CYP11A1;Cypxia1;Cytochrome P450 11A1;Cytochrome P450(scc;P450(scc);P450scc;

Gene names

Cyp11a1

Research area

Cardiovascular

Target protein

CYP11A1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1907Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.287 2.182 2.142
2.077
600ng/L 1.867 1.842 1.802
1.816
300ng/L 1.22 1.207 1.167
1.194
150ng/L 0.748 0.735 0.695
0.721
75ng/L 0.394 0.407 0.367
0.42
0ng/L 0.039 0.04 0
0.041

Precision

We measured random samples of E1907Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 203.96 11.65 5.71
Intra-Assay 2 18 326.85 12.75 3.9
Intra-Assay 3 18 97.71 4.62 4.73

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1907Ra

    $320.00

  • 96T

    E1907Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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