Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Mouse Cd82 Antigen, CD82 ELISA Kit

Mouse Cd82 Antigen, CD82 ELISA Kit

Mouse Cd82 Antigen, CD82 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.55ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 1.55ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

CD82 antigen

Code

E1922Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.55ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

CD82 (Cluster of Differentiation 82), or KAI1, is a human protein encoded by the CD82 gene. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the tetraspanin/transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt accession

MASS(Da)

29,629

GeneID

12521

Synonyms

AL023070;C33;C33 antigen;CD antigen CD82;CD82;CD82 antigen;IA4;Inducible membrane protein R2;Ka;Kai1;Metastasis suppressor Kangai-1 homolog;Tsp;Tspan27;

Gene names

Cd82

Research area

Immunology; Epigenetics and Nuclear Signaling; Cancer

Target protein

CD82

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1922Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
480ng/L 2.134 2.055 2.018
1.977
240ng/L 1.366 1.362 1.324
1.358
120ng/L 0.602 0.688 0.651
0.774
60ng/L 0.385 0.407 0.369
0.428
30ng/L 0.154 0.179 0.142
0.205
0ng/L 0.046 0.038 0
0.029

Precision

We measured random samples of E1922Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 331.73 15.12 4.56
Intra-Assay 2 18 196.48 9.12 4.64
Intra-Assay 3 18 114.56 7.25 6.32

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E1922Mo

    $320.00

  • 96T

    E1922Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up