Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2075Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.199 | 2.215 | 2.169 |
| 2.231 | |||
| 12ng/mL | 1.369 | 1.292 | 1.247 |
| 1.216 | |||
| 6ng/mL | 0.714 | 0.757 | 0.711 |
| 0.8 | |||
| 3ng/mL | 0.465 | 0.422 | 0.377 |
| 0.379 | |||
| 1.5ng/mL | 0.235 | 0.237 | 0.191 |
| 0.239 | |||
| 0ng/mL | 0.061 | 0.046 | 0 |
| 0.03 |
Precision
We measured random samples of E2075Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 11.3 | 0.71 | 6.27 |
| Intra-Assay | 2 | 18 | 17.13 | 0.56 | 3.25 |
| Intra-Assay | 3 | 18 | 4.23 | 0.21 | 4.89 |
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