Human Endoglin, ENG ELISA Kit

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Home > Product > ELISA Kit > Human Endoglin, ENG ELISA Kit

Human Endoglin, ENG ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.16ng/ml

Detection range: 0.3-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Endoglin

Code

E3407Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.16ng/ml

Detection range

0.3-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed: 21737454, PubMed: 23300529). Required for normal structure and integrity of adult vasculature (PubMed: 7894484). Regulates the migration of vascular endothelial cells (PubMed: 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed: 1692830). Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed: 8370410, PubMed: 21737454, PubMed: 22347366, PubMed: 23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed: 21737454, PubMed: 22347366, PubMed: 23300529).

UniProt accession

P17813

MASS（Da）

70,578

GeneID

2022

Synonyms

CD antigen CD105;Endoglin;ENG

Gene names

ENG

Research area

Cancer

Target protein

ENG

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E3407Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	1.879	1.865	1.824
	1.851
20ng/ml	0.896	0.906	0.865
	0.916
10ng/ml	0.546	0.515	0.474
	0.485
5ng/ml	0.269	0.279	0.238
	0.289
2.5ng/ml	0.09	0.098	0.057
	0.106
0ng/ml	0.029	0.041	0
	0.053

Precision

We measured random samples of E3407Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	11.2	0.74	6.6
Intra-Assay	2	18	26	1.17	4.5
Intra-Assay	3	18	34.4	0.83	2.4

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E3407Hu

$320.00
96T

E3407Hu

$458.00

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Lead time: Within two weeks

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