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Home > Product > ELISA Kit > Bovine Hydroxymethylglutaryl-CoA Lyase, HMGCL ELISA Kit

Bovine Hydroxymethylglutaryl-CoA Lyase, HMGCL ELISA Kit

Bovine Hydroxymethylglutaryl-CoA Lyase, HMGCL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.26ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 48T,96T

Sensitivity: 7.26ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Hydroxymethylglutaryl-CoA lyase

Code

E2217Bo

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.26ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.

UniProt accession

MASS(Da)

34,168

GeneID

317658

Synonyms

3-hydroxy-3-methylglutarate-CoA lyase;HL;HMGCL;HMG-CoA lyase;Hydroxymethylglutaryl-CoA lyase, mitochondrial

Gene names

HMGCL

Research area

Others

Target protein

HMGCL

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2217Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.251 2.239 2.182
2.228
800ng/L 1.824 1.78 1.722
1.736
400ng/L 1.106 1.146 1.089
1.186
200ng/L 0.655 0.694 0.637
0.733
100ng/L 0.379 0.371 0.313
0.362
0ng/L 0.06 0.058 0
0.055

Precision

We measured random samples of E2217Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 131.54 6.38 4.85
Intra-Assay 2 18 621.54 32.94 5.3
Intra-Assay 3 18 434.51 30.25 6.96

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2217Bo

    $320.00

  • 96T

    E2217Bo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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