Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2268Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 120ng/mL | 2.316 | 2.345 | 2.282 |
| 2.374 | |||
| 60ng/mL | 1.421 | 1.438 | 1.375 |
| 1.454 | |||
| 30ng/mL | 0.847 | 0.779 | 0.717 |
| 0.712 | |||
| 15ng/mL | 0.468 | 0.478 | 0.416 |
| 0.488 | |||
| 7.5ng/mL | 0.268 | 0.265 | 0.203 |
| 0.262 | |||
| 0ng/mL | 0.075 | 0.063 | 0 |
| 0.05 |
Precision
We measured random samples of E2268Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 55.3 | 1.86 | 3.37 |
| Intra-Assay | 2 | 18 | 32.73 | 1.71 | 5.21 |
| Intra-Assay | 3 | 18 | 25.47 | 1.58 | 6.19 |
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