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Home > Product > ELISA Kit > Human Acid Ceramidase, ASAH1 ELISA Kit

Human Acid Ceramidase, ASAH1 ELISA Kit

Human Acid Ceramidase, ASAH1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-180ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.28ng/ml

Detection range: 0.5-180ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Acid Ceramidase

Code

E3476Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.28ng/ml

Detection range

0.5-180ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (PubMed: 10610716, PubMed: 7744740, PubMed: 15655246, PubMed: 11451951). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed: 10610716). Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (PubMed: 7744740, PubMed: 15655246). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed: 12764132, PubMed: 12815059). For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (PubMed: 12764132). Has also an N-acylethanolamine hydrolase activity (PubMed: 15655246). By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (PubMed: 17713573). Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (PubMed: 22261821). [Isoform 2]: May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity.

UniProt accession

MASS(Da)

44,660

GeneID

427

Synonyms

AC;ACDase;Acid CDase;Acid ceramidase;Acid ceramidase subunit alpha;Acid ceramidase subunit beta;Acylsphingosine deacylase;ASAH 1;ASAH1;N-acylethanolamine hydrolase ASAH1;N-acylsphingosine amidohydrolase;PHP32;Putative 32 kDa heart protein;

Gene names

ASAH1

Research area

Others

Target protein

ASAH1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3476Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
100ng/ml 2.257 2.193 2.14
2.129
50ng/ml 1.553 1.511 1.458
1.469
25ng/ml 0.773 0.749 0.696
0.726
12.5ng/ml 0.5 0.449 0.396
0.398
6.25ng/ml 0.314 0.295 0.242
0.276
0ng/ml 0.057 0.053 0
0.05

Precision

We measured random samples of E3476Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 31 1.46 4.7
Intra-Assay 2 18 65.9 2.89 4.4
Intra-Assay 3 18 12.7 0.57 4.5

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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