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Home > Product > ELISA Kit > Mouse Pou Domain, Class 5, Transcription Factor 1, POU5F1 ELISA Kit

Mouse Pou Domain, Class 5, Transcription Factor 1, POU5F1 ELISA Kit

Mouse Pou Domain, Class 5, Transcription Factor 1, POU5F1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.52ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 48T,96T

Sensitivity: 9.52ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

POU domain, class 5, transcription factor 1

Code

E2357Mo

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.52ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1972777, PubMed:1690859, PubMed:1967980, PubMed:17525163, PubMed:23376973). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:15863505, PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1972777, PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:19740739, PubMed:29153991, PubMed:23376973).

UniProt accession

MASS(Da)

38,216

GeneID

18999

Synonyms

44472;44473;NF-A3;Oct;Oct-;Oct3/;Oct-3/;Oct3/4;Oct-3/4;Octamer-binding protein 3;Octamer-binding protein 4;Octamer-binding transcription factor 3;Otf;Otf-;Otf3-;Otf-3;Otf3g;Otf3-rs7;Otf4;Otf-4;POU domain, class 5, transcription factor 1;POU5F1;

Gene names

Pou5f1

Research area

Others

Target protein

POU5F1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2357Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.366 2.268 2.228
2.171
1600ng/L 1.373 1.386 1.345
1.398
800ng/L 0.701 0.7 0.66
0.699
400ng/L 0.469 0.429 0.388
0.388
200ng/L 0.19 0.228 0.188
0.267
0ng/L 0.047 0.04 0
0.033

Precision

We measured random samples of E2357Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 869.4 43.09 4.96
Intra-Assay 2 18 1535.42 56.91 3.71
Intra-Assay 3 18 584.33 35.06 6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2357Mo

    $320.00

  • 96T

    E2357Mo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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