Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2379Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.275 | 2.202 | 2.144 |
| 2.129 | |||
| 12ng/mL | 1.128 | 1.172 | 1.115 |
| 1.217 | |||
| 6ng/mL | 0.748 | 0.76 | 0.702 |
| 0.772 | |||
| 3ng/mL | 0.471 | 0.486 | 0.428 |
| 0.501 | |||
| 1.5ng/mL | 0.194 | 0.196 | 0.138 |
| 0.199 | |||
| 0ng/mL | 0.055 | 0.058 | 0 |
| 0.061 |
Precision
We measured random samples of E2379Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.42 | 0.16 | 6.68 |
| Intra-Assay | 2 | 18 | 16.95 | 0.61 | 3.59 |
| Intra-Assay | 3 | 18 | 11.76 | 0.63 | 5.32 |
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