Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2434Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 2.032 | 2.176 | 2.12 |
| 2.32 | |||
| 4ng/ml | 1.382 | 1.349 | 1.293 |
| 1.316 | |||
| 2ng/ml | 0.728 | 0.793 | 0.737 |
| 0.858 | |||
| 1ng/ml | 0.46 | 0.493 | 0.437 |
| 0.526 | |||
| 0.5ng/ml | 0.266 | 0.244 | 0.189 |
| 0.223 | |||
| 0ng/ml | 0.053 | 0.056 | 0 |
| 0.059 |
Precision
We measured random samples of E2434Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3.97 | 0.13 | 3.37 |
| Intra-Assay | 2 | 18 | 0.89 | 0.06 | 6.17 |
| Intra-Assay | 3 | 18 | 2.44 | 0.14 | 5.6 |
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