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Home > Product > ELISA Kit > Human Neprilysin, NEP ELISA Kit

Human Neprilysin, NEP ELISA Kit

Human Neprilysin, NEP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.15ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 14.15ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neprilysin

Code

E3564Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.15ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed: 15283675, PubMed: 8168535). Biologically important in the destruction of opioid Peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed: 17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed: 15283675). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed: 2531377, PubMed: 2972276, PubMed: 16254193). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed: 20876573).

UniProt accession

MASS(Da)

85,514

GeneID

4311

Synonyms

Atriopeptidase;CALLA;CD antigen CD10;Common acute lymphocytic leukemia antigen;Enkephalinase;MME;NEP;Neprilysin;Neutral endopeptidase;Neutral endopeptidase 24.11;SFE;Skin fibroblast elastase

Gene names

MME

Research area

Neuroscience

Target protein

MME

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3564Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.132 2.181 2.124
2.23
2400ng/L 1.32 1.336 1.279
1.352
1200ng/L 0.758 0.733 0.676
0.707
600ng/L 0.45 0.502 0.446
0.555
300ng/L 0.242 0.284 0.227
0.325
0ng/L 0.049 0.057 0
0.065

Precision

We measured random samples of E3564Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1495.2 74.3 5
Intra-Assay 2 18 3565.4 99.12 2.8
Intra-Assay 3 18 447.8 23.65 5.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3564Hu

    $320.00

  • 96T

    E3564Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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