Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2649Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.311 | 2.271 | 2.224 |
| 2.231 | |||
| 1200ng/L | 1.278 | 1.262 | 1.215 |
| 1.247 | |||
| 600ng/L | 0.704 | 0.709 | 0.662 |
| 0.713 | |||
| 300ng/L | 0.413 | 0.465 | 0.418 |
| 0.517 | |||
| 150ng/L | 0.266 | 0.265 | 0.218 |
| 0.264 | |||
| 0ng/L | 0.046 | 0.047 | 0 |
| 0.047 |
Precision
We measured random samples of E2649Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 891.36 | 49.27 | 5.53 |
| Intra-Assay | 2 | 18 | 1505.98 | 50.08 | 3.33 |
| Intra-Assay | 3 | 18 | 397.79 | 29.85 | 7.5 |
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