Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2732Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 1.901 | 1.809 | 1.759 |
| 1.718 | |||
| 800ng/L | 1.112 | 1.042 | 0.991 |
| 0.971 | |||
| 400ng/L | 0.591 | 0.511 | 0.461 |
| 0.431 | |||
| 200ng/L | 0.153 | 0.193 | 0.143 |
| 0.234 | |||
| 100ng/L | 0.063 | 0.074 | 0.024 |
| 0.085 | |||
| 0ng/L | 0.041 | 0.051 | 0 |
| 0.06 |
Precision
We measured random samples of E2732Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 403.6 | 15.88 | 3.93 |
| Intra-Assay | 2 | 18 | 1359.63 | 52.86 | 3.89 |
| Intra-Assay | 3 | 18 | 902.57 | 46.19 | 5.12 |
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