Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2863Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4000ng/L | 1.689 | 1.836 | 1.792 |
| 1.983 | |||
| 2000ng/L | 0.899 | 0.913 | 0.869 |
| 0.926 | |||
| 1000ng/L | 0.552 | 0.521 | 0.477 |
| 0.491 | |||
| 500ng/L | 0.191 | 0.228 | 0.184 |
| 0.266 | |||
| 250ng/L | 0.152 | 0.114 | 0.07 |
| 0.076 | |||
| 0ng/L | 0.062 | 0.044 | 0 |
| 0.026 |
Precision
We measured random samples of E2863Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3538.37 | 98.73 | 2.79 |
| Intra-Assay | 2 | 18 | 1329.58 | 51.56 | 3.88 |
| Intra-Assay | 3 | 18 | 1619.11 | 69.81 | 4.31 |
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