Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2864Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 2.545 | 2.464 | 2.428 |
| 2.383 | |||
| 1000ng/L | 1.592 | 1.559 | 1.523 |
| 1.527 | |||
| 500ng/L | 0.907 | 0.857 | 0.821 |
| 0.807 | |||
| 250ng/L | 0.514 | 0.455 | 0.418 |
| 0.395 | |||
| 125ng/L | 0.258 | 0.234 | 0.197 |
| 0.21 | |||
| 0ng/L | 0.045 | 0.036 | 0 |
| 0.028 |
Precision
We measured random samples of E2864Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 875.78 | 28.76 | 3.28 |
| Intra-Assay | 2 | 18 | 287.87 | 13.63 | 4.74 |
| Intra-Assay | 3 | 18 | 510.68 | 28.81 | 5.64 |
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