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Home > Product > ELISA Kit > Human Carbohydrate Sulfotransferase 7, CHST7 ELISA Kit

Human Carbohydrate Sulfotransferase 7, CHST7 ELISA Kit

Human Carbohydrate Sulfotransferase 7, CHST7 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Carbohydrate Sulfotransferase 7

Code

E2876Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc.

UniProt accession

MASS(Da)

54,266

GeneID

56548

Synonyms

C6ST-2;Carbohydrate sulfotransferase 7;Chondroitin 6-sulfotransferase 2;CHST7;Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5;GlcNAc6ST-4;Gn6st-4;GST-5;N-acetylglucosamine 6-O-sulfotransferase 4;

Gene names

CHST7

Research area

Signal Transduction

Target protein

CHST7

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2876Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 1.961 1.956 1.913
1.952
4ng/ml 0.882 0.917 0.873
0.952
2ng/ml 0.431 0.431 0.388
0.431
1ng/ml 0.185 0.239 0.195
0.292
0.5ng/ml 0.134 0.129 0.086
0.125
0ng/ml 0.061 0.043 0
0.026

Precision

We measured random samples of E2876Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.67 0.13 2.25
Intra-Assay 2 18 3.94 0.15 3.75
Intra-Assay 3 18 2.05 0.11 5.12

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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