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Home > Product > ELISA Kit > Human Centromere-associated Protein E, CENPE ELISA Kit

Human Centromere-associated Protein E, CENPE ELISA Kit

Human Centromere-associated Protein E, CENPE ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.049ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.049ng/ml

Detection range: 0.1-35ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Centromere-associated protein E

Code

E2885Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.049ng/ml

Detection range

0.1-35ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed:7889940, PubMed:23891108, PubMed:25395579). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed:25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed:23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed:25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed:17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).

UniProt accession

MASS(Da)

316,415

GeneID

1062

Synonyms

CENPE;CENP-E;Centromere protein E;Centromere-associated protein E;KIF10;Kinesin-7;Kinesin-related protein CENPE;MCPH13;PPP1R61;

Gene names

CENPE

Research area

Epigenetics and Nuclear Signaling

Target protein

CENPE

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Centromere-associated Protein E, CENPE ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.137 2.143 2.093
2.15
10ng/ml 1.279 1.348 1.297
1.416
5ng/ml 0.836 0.831 0.78
0.826
2.5ng/ml 0.443 0.459 0.408
0.475
1.25ng/ml 0.297 0.294 0.243
0.291
0ng/ml 0.051 0.051 0
0.051

Precision

We measured random samples of E2885Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.16 0.49 6.88
Intra-Assay 2 18 10.95 0.43 3.89
Intra-Assay 3 18 3.74 0.23 6.12

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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