Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2951Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 800ng/L | 2.681 | 2.535 | 2.485 |
| 2.389 | |||
| 400ng/L | 1.575 | 1.535 | 1.485 |
| 1.496 | |||
| 200ng/L | 0.826 | 0.791 | 0.741 |
| 0.757 | |||
| 100ng/L | 0.468 | 0.5 | 0.45 |
| 0.532 | |||
| 50ng/L | 0.251 | 0.232 | 0.182 |
| 0.213 | |||
| 0ng/L | 0.065 | 0.05 | 0 |
| 0.035 |
Precision
We measured random samples of E2951Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 382.28 | 13.69 | 3.58 |
| Intra-Assay | 2 | 18 | 177.02 | 9.17 | 5.18 |
| Intra-Assay | 3 | 18 | 163.24 | 7.39 | 4.52 |
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