Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Rho Guanine Nucleotide Exchange Factor 4, ARHGEF4 ELISA Kit

Human Rho Guanine Nucleotide Exchange Factor 4, ARHGEF4 ELISA Kit

Human Rho Guanine Nucleotide Exchange Factor 4, ARHGEF4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.14ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.14ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rho Guanine Nucleotide Exchange Factor 4

Code

E3237Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.14ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.

UniProt accession

MASS(Da)

79,067

GeneID

50649

Synonyms

APC-stimulated guanine nucleotide exchange factor 1;ARHGEF4;ASEF;ASEF1;GEF4;Rho guanine nucleotide exchange factor 4;SMIM39;STM6

Gene names

ARHGEF4

Research area

Others

Target protein

ARHGEF4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3237Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.63 2.643 2.599
2.657
320ng/L 1.371 1.384 1.34
1.398
160ng/L 0.866 0.818 0.773
0.77
80ng/L 0.532 0.481 0.436
0.43
40ng/L 0.258 0.257 0.212
0.256
0ng/L 0.06 0.045 0
0.029

Precision

We measured random samples of E3237Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 133.18 7.61 5.71
Intra-Assay 2 18 332.05 10.07 3.03
Intra-Assay 3 18 244.38 13.11 5.37

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E3237Hu

    $320.00

  • 96T

    E3237Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up