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Home > Product > ELISA Kit > Human Interferon Regulatory Factor 4, IRF4 ELISA Kit

Human Interferon Regulatory Factor 4, IRF4 ELISA Kit

Human Interferon Regulatory Factor 4, IRF4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.58ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.58ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

interferon regulatory factor 4

Code

E3334Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.58ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).

UniProt accession

MASS(Da)

51,772

GeneID

3662

Synonyms

Interferon regulatory factor 4;IRF4;IRF-4;LSIRF;Lymphocyte-specific interferon regulatory factor;Multiple myeloma oncogene 1;MUM1;NF-EM5;SHEP8;

Gene names

IRF4

Research area

Tags & Cell Markers; Epigenetics and Nuclear Signaling; Cancer

Target protein

IRF4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3334Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.106 2.095 2.06
2.084
600ng/L 1.506 1.539 1.505
1.572
300ng/L 0.603 0.603 0.569
0.604
150ng/L 0.396 0.419 0.385
0.442
75ng/L 0.172 0.171 0.136
0.17
0ng/L 0.026 0.034 0
0.042

Precision

We measured random samples of E3334Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 326.33 10.86 3.33
Intra-Assay 2 18 528.39 14.8 2.8
Intra-Assay 3 18 245.97 9.66 3.93

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3334Hu

    $320.00

  • 96T

    E3334Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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