Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3846Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 20ng/ml | 1.976 | 1.962 | 1.888 |
| 1.947 | |||
| 10ng/ml | 1.129 | 1.159 | 1.085 |
| 1.189 | |||
| 5ng/ml | 0.611 | 0.63 | 0.556 |
| 0.649 | |||
| 2.5ng/ml | 0.309 | 0.326 | 0.253 |
| 0.344 | |||
| 1.25ng/ml | 0.157 | 0.152 | 0.079 |
| 0.148 | |||
| 0ng/ml | 0.08 | 0.074 | 0 |
| 0.067 |
Precision
We measured random samples of E3846Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 15.2 | 0.5 | 3.4 |
| Intra-assay | 2 | 18 | 7.2 | 0.4 | 5.4 |
| Intra-assay | 3 | 18 | 6.3 | 0.3 | 4.3 |
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