Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4039hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 32ng/ml | 2.145 | 2.117 | 2.086 |
| 2.088 | |||
| 16ng/ml | 1.48 | 1.408 | 1.378 |
| 1.336 | |||
| 8ng/ml | 0.725 | 0.733 | 0.702 |
| 0.741 | |||
| 4ng/ml | 0.326 | 0.351 | 0.32 |
| 0.376 | |||
| 2ng/ml | 0.188 | 0.155 | 0.125 |
| 0.122 | |||
| 0ng/ml | 0.021 | 0.031 | 0 |
| 0.04 |
Precision
We measured random samples of E4039hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 11.85 | 0.73 | 6.17 |
| Intra-Assay | 2 | 18 | 24.05 | 1.28 | 5.31 |
| Intra-Assay | 3 | 18 | 5.76 | 0.27 | 4.66 |
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