Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4213hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 2.23 | 2.266 | 2.228 |
| 2.302 | |||
| 1000ng/L | 1.354 | 1.266 | 1.228 |
| 1.178 | |||
| 500ng/L | 0.773 | 0.736 | 0.698 |
| 0.698 | |||
| 250ng/L | 0.534 | 0.503 | 0.465 |
| 0.473 | |||
| 125ng/L | 0.17 | 0.21 | 0.172 |
| 0.251 | |||
| 0ng/L | 0.051 | 0.038 | 0 |
| 0.025 |
Precision
We measured random samples of E4213hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 385.91 | 32.14 | 8.33 |
| Intra-Assay | 2 | 18 | 958.12 | 35.39 | 3.69 |
| Intra-Assay | 3 | 18 | 935.01 | 47.11 | 5.04 |
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