Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E5294Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.363	2.52	2.475
	2.676
800ng/L	1.537	1.481	1.436
	1.426
400ng/L	0.843	0.835	0.79
	0.827
200ng/L	0.514	0.499	0.454
	0.485
100ng/L	0.261	0.259	0.214
	0.258
0ng/L	0.047	0.045	0
	0.043

We measured random samples of E5294Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	594.15	34.07	5.74
Intra-Assay	2	18	688.07	20.59	2.99
Intra-Assay	3	18	141.22	10.25	7.26

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