Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5787Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 640ng/L | 2.394 | 2.49 | 2.445 |
| 2.585 | |||
| 320ng/L | 1.417 | 1.46 | 1.415 |
| 1.503 | |||
| 160ng/L | 0.769 | 0.814 | 0.769 |
| 0.86 | |||
| 80ng/L | 0.416 | 0.458 | 0.413 |
| 0.499 | |||
| 40ng/L | 0.203 | 0.204 | 0.159 |
| 0.206 | |||
| 0ng/L | 0.052 | 0.045 | 0 |
| 0.038 |
Precision
We measured random samples of E5787Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 196.98 | 10.83 | 5.5 |
| Intra-Assay | 2 | 18 | 430.15 | 15.57 | 3.62 |
| Intra-Assay | 3 | 18 | 76.48 | 3.5 | 4.57 |
Protocols
References
Customer reviews and Q&As
