Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E5354Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.14	2.121	2.078
	2.102
24ng/mL	1.52	1.563	1.52
	1.606
12ng/mL	1.023	1.096	1.053
	1.169
6ng/mL	0.732	0.723	0.68
	0.714
3ng/mL	0.404	0.408	0.365
	0.413
0ng/mL	0.027	0.043	0
	0.059

We measured random samples of E5354Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	16.56	0.93	5.61
Intra-Assay	2	18	5.57	0.36	6.55
Intra-Assay	3	18	9.65	0.62	6.43

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