Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5536Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12.8ng/mL | 2.14 | 2.246 | 2.205 |
| 2.351 | |||
| 6.4ng/mL | 1.44 | 1.336 | 1.295 |
| 1.232 | |||
| 3.2ng/mL | 0.64 | 0.673 | 0.632 |
| 0.705 | |||
| 1.6ng/mL | 0.446 | 0.396 | 0.355 |
| 0.346 | |||
| 0.8ng/mL | 0.215 | 0.19 | 0.15 |
| 0.165 | |||
| 0ng/mL | 0.046 | 0.041 | 0 |
| 0.036 |
Precision
We measured random samples of E5536Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.37 | 0.28 | 3.35 |
| Intra-Assay | 2 | 18 | 4.58 | 0.23 | 5.06 |
| Intra-Assay | 3 | 18 | 1.85 | 0.08 | 4.13 |
Protocols
References
Customer reviews and Q&As
