Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5607Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.267 | 2.214 | 2.161 |
| 2.161 | |||
| 600ng/L | 1.373 | 1.344 | 1.291 |
| 1.315 | |||
| 300ng/L | 0.756 | 0.752 | 0.7 |
| 0.749 | |||
| 150ng/L | 0.439 | 0.46 | 0.407 |
| 0.48 | |||
| 75ng/L | 0.238 | 0.255 | 0.203 |
| 0.273 | |||
| 0ng/L | 0.051 | 0.053 | 0 |
| 0.054 |
Precision
We measured random samples of E5607Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 573.09 | 11.29 | 1.97 |
| Intra-Assay | 2 | 18 | 183.59 | 11.19 | 6.1 |
| Intra-Assay | 3 | 18 | 454.37 | 33.25 | 7.32 |
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