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Home > Product > ELISA Kit > Human Cerebellin-1, CBLN1 ELISA Kit

Human Cerebellin-1, CBLN1 ELISA Kit

Human Cerebellin-1, CBLN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.34ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.34ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cerebellin-1

Code

E3808Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.34ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the matching and maintenance of pre- and post-synaptic elements at parallel fiber-Purkinje cell synapses, the establishment of the proper pattern of climbing fiber-Purkinje cell innervation, and induction of long-term depression at parallel fiber-Purkinje cell synapses. Plays a role as a synaptic organizer that acts bidirectionally on both pre- and post-synaptic components. On the one hand induces accumulation of synaptic vesicles in the pre-synaptic part by binding with NRXN1 and in other hand induces clustering of GRID2 and its associated proteins at the post-synaptic site through association of GRID2. NRXN1-CBLN1-GRID2 complex directly induces parallel fiber protrusions that encapsulate spines of Purkinje cells leading to accumulation of GRID2 and synaptic vesicles. Required for CBLN3 export from the endoplasmic reticulum and secretion (By similarity). NRXN1-CBLN1-GRID2 complex mediates the D-Serine-dependent long term depression signals and AMPA receptor endocytosis (PubMed: 27418511). Essential for long-term maintenance but not establishment of excitatory synapses (By similarity). Inhibits the formation and function of inhibitory GABAergic synapses in cerebellar Purkinje cells (By similarity). The cerebellin Peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release (By similarity).

UniProt accession

MASS(Da)

21,097

GeneID

869

Synonyms

[des-Ser1]-cerebellin;CBLN 1;CBLN1;CER;Cerebellin;Cerebellin-1;Precerebellin

Gene names

CBLN1

Research area

Others

Target protein

CBLN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3808Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.15 2.112 2.055
2.074
400ng/L 1.493 1.51 1.453
1.527
200ng/L 0.885 0.872 0.815
0.859
100ng/L 0.486 0.456 0.398
0.425
50ng/L 0.244 0.277 0.22
0.31
0ng/L 0.071 0.057 0
0.044

Precision

We measured random samples of E3808Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 195.5 11.14 5.7
Intra-Assay 2 18 341.7 14.3 4.2
Intra-Assay 3 18 124.4 5.86 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3808Hu

    $320.00

  • 96T

    E3808Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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