Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5902Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.379 | 2.239 | 2.192 |
| 2.099 | |||
| 800ng/L | 1.334 | 1.323 | 1.276 |
| 1.312 | |||
| 400ng/L | 0.711 | 0.77 | 0.723 |
| 0.828 | |||
| 200ng/L | 0.496 | 0.485 | 0.438 |
| 0.475 | |||
| 100ng/L | 0.232 | 0.226 | 0.179 |
| 0.22 | |||
| 0ng/L | 0.043 | 0.047 | 0 |
| 0.051 |
Precision
We measured random samples of E5902Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 913.7 | 28.48 | 3.12 |
| Intra-Assay | 2 | 18 | 519.44 | 31 | 5.97 |
| Intra-Assay | 3 | 18 | 262.96 | 15.42 | 5.87 |
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