Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5961Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 1.888 | 1.931 | 1.885 |
| 1.973 | |||
| 800ng/L | 0.89 | 0.986 | 0.941 |
| 1.083 | |||
| 400ng/L | 0.477 | 0.456 | 0.41 |
| 0.434 | |||
| 200ng/L | 0.286 | 0.291 | 0.245 |
| 0.296 | |||
| 100ng/L | 0.147 | 0.132 | 0.087 |
| 0.117 | |||
| 0ng/L | 0.05 | 0.045 | 0 |
| 0.041 |
Precision
We measured random samples of E5961Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1077.54 | 26.08 | 2.42 |
| Intra-Assay | 2 | 18 | 327.87 | 14.65 | 4.47 |
| Intra-Assay | 3 | 18 | 830.01 | 32.94 | 3.97 |
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