Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6003Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.356 | 2.328 | 2.276 |
| 2.3 | |||
| 1200ng/L | 1.273 | 1.29 | 1.238 |
| 1.307 | |||
| 600ng/L | 0.67 | 0.715 | 0.663 |
| 0.759 | |||
| 300ng/L | 0.501 | 0.45 | 0.399 |
| 0.399 | |||
| 150ng/L | 0.259 | 0.225 | 0.173 |
| 0.192 | |||
| 0ng/L | 0.053 | 0.052 | 0 |
| 0.051 |
Precision
We measured random samples of E6003Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 945.82 | 50.72 | 5.36 |
| Intra-Assay | 2 | 18 | 1231.12 | 38.06 | 3.09 |
| Intra-Assay | 3 | 18 | 409.53 | 23.39 | 5.71 |
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