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Home > Product > ELISA Kit > Human Putative Pre-MRNA-Splicing Factor Atp-dependent Rna Helicase Dhx32, DHX32 ELISA Kit

Human Putative Pre-MRNA-Splicing Factor Atp-dependent Rna Helicase Dhx32, DHX32 ELISA Kit

Human Putative Pre-MRNA-Splicing Factor Atp-dependent Rna Helicase Dhx32, DHX32 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.48ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.48ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Code

E6042Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.48ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 is an enzyme that in humans is encoded by the DHX32 gene. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates two transcript variants, but the full length nature of one of the variants has not been defined.

UniProt accession

MASS(Da)

84,419

GeneID

55760

Synonyms

DDX32;DEAD/H box 32;DEAD/H helicase-like protein 1;DEAH box protein 32;DHLP1;DHX32;HuDDX32;Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Gene names

DHX32

Research area

Others

Target protein

DHX32

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6042Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.34 2.311 2.26
2.282
1200ng/L 1.488 1.551 1.5
1.614
600ng/L 1.023 1.077 1.025
1.13
300ng/L 0.737 0.733 0.682
0.729
150ng/L 0.405 0.426 0.375
0.446
0ng/L 0.062 0.051 0
0.04

Precision

We measured random samples of E6042Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1215.53 57.7 4.75
Intra-Assay 2 18 240.66 15.08 6.27
Intra-Assay 3 18 748.1 47.12 6.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E6042Hu

    $320.00

  • 96T

    E6042Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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