Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6321Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.273 | 2.201 | 2.137 |
| 2.129 | |||
| 600ng/L | 1.469 | 1.382 | 1.317 |
| 1.294 | |||
| 300ng/L | 0.895 | 0.876 | 0.812 |
| 0.857 | |||
| 150ng/L | 0.549 | 0.536 | 0.472 |
| 0.524 | |||
| 75ng/L | 0.248 | 0.284 | 0.22 |
| 0.32 | |||
| 0ng/L | 0.069 | 0.064 | 0 |
| 0.059 |
Precision
We measured random samples of E6321Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 443.75 | 27.83 | 6.27 |
| Intra-Assay | 2 | 18 | 557.82 | 15.33 | 2.75 |
| Intra-Assay | 3 | 18 | 142.87 | 8.18 | 5.72 |
Protocols
References
Customer reviews and Q&As
