Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Ubiquitin Thioesterase Otub2, OTUB2 ELISA Kit

Human Ubiquitin Thioesterase Otub2, OTUB2 ELISA Kit

Human Ubiquitin Thioesterase Otub2, OTUB2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.31ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.31ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ubiquitin thioesterase OTUB2

Code

E6374Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.31ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

UniProt accession

MASS(Da)

27,213

GeneID

78990

Synonyms

C14orf137;Deubiquitinating enzyme OTUB2;OTB2;OTU domain-containing ubiquitin aldehyde-binding protein 2;OTU2;OTUB2;Otubain-2;Ubiquitin thioesterase OTUB2;Ubiquitin-specific-processing protease OTUB2;

Gene names

OTUB2

Research area

Cell Biology

Target protein

OTUB2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6374Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.852 1.855 1.827
1.858
800ng/L 0.992 0.965 0.937
0.938
400ng/L 0.647 0.571 0.543
0.494
200ng/L 0.147 0.188 0.16
0.229
100ng/L 0.063 0.059 0.031
0.055
0ng/L 0.028 0.028 0
0.028

Precision

We measured random samples of E6374Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 968.43 49.45 5.11
Intra-Assay 2 18 1362.78 42.61 3.13
Intra-Assay 3 18 351.09 17.97 5.12

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E6374Hu

    $320.00

  • 96T

    E6374Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up