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Home > Product > ELISA Kit > Human Biogenesis of Lysosome-related Organelles Complex 1 Subunit 1, BLOC1S1 ELISA Kit

Human Biogenesis of Lysosome-related Organelles Complex 1 Subunit 1, BLOC1S1 ELISA Kit

Human Biogenesis of Lysosome-related Organelles Complex 1 Subunit 1, BLOC1S1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.68ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.68ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Biogenesis of lysosome-related organelles complex 1 subunit 1

Code

E6559Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.68ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension (PubMed:17182842). As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PubMed:25898167). May negatively regulate aerobic respiration through mitochondrial protein lysine-acetylation. May counteract the action of the deacetylase SIRT3 by acetylating and regulating proteins of the mitochondrial respiratory chain including ATP5F1A and NDUFA9.

UniProt accession

MASS(Da)

17,263

GeneID

2647

Synonyms

Biogenesis of lysosome-related organelles complex 1 subunit 1;BLOC-1 subunit 1;BLOC1S1;BLOS1;BORCS1;GCN5L1;GCN5-like protein 1;MICoA;Protein RT14;RT14

Gene names

BLOC1S1

Research area

Cardiovascular; Signal Transduction

Target protein

BLOC1S1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6559Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.985 2.045 2.02
2.105
600ng/L 1.427 1.421 1.396
1.415
300ng/L 0.628 0.594 0.569
0.559
150ng/L 0.383 0.367 0.343
0.352
75ng/L 0.167 0.189 0.164
0.211
0ng/L 0.026 0.025 0
0.023

Precision

We measured random samples of E6559Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 871.76 44.12 5.06
Intra-Assay 2 18 524.19 27.56 5.26
Intra-Assay 3 18 295.61 12.61 4.27

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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