Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E6671Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 2.538 | 2.492 | 2.453 |
| 2.445 | |||
| 4ng/ml | 1.513 | 1.513 | 1.474 |
| 1.513 | |||
| 2ng/ml | 0.771 | 0.827 | 0.788 |
| 0.883 | |||
| 1ng/ml | 0.472 | 0.461 | 0.423 |
| 0.45 | |||
| 0.5ng/ml | 0.213 | 0.243 | 0.205 |
| 0.273 | |||
| 0ng/ml | 0.031 | 0.039 | 0 |
| 0.046 |
Precision
We measured random samples of E6671Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.73 | 0.13 | 4.7 |
| Intra-Assay | 2 | 18 | 5.08 | 0.16 | 3.06 |
| Intra-Assay | 3 | 18 | 1.49 | 0.1 | 6.47 |
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