Human Methionine Synthase, MTR ELISA Kit

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Home > Product > ELISA Kit > Human Methionine Synthase, MTR ELISA Kit

Human Methionine Synthase, MTR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.027ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Methionine Synthase

Code

E3942Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.027ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the transfer of a methyl group from methylcob(III)alamin (MeCbl) to homocysteine, yielding enzyme-bound cob(I)alamin and methionine in the cytosol (PubMed: 27771510). MeCbl is an active form of cobalamin (vitamin B12) used as a cofactor for methionine biosynthesis. Cob(I)alamin form is regenerated to MeCbl by a transfer of a methyl group from 5-methyltetrahydrofolate (PubMed: 27771510). The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine Synthase reductase) and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine (PubMed: 27771510).

UniProt accession

Q99707

MASS（Da）

140,527

GeneID

4548

Synonyms

5-methyltetrahydrofolate--homocysteine methyltransferase;Cobalamin-dependent methionine Synthase;Methionine Synthase;MS;MTR;Vitamin-B12 dependent methionine Synthase

Gene names

MTR

Research area

Metabolism

Target protein

MTR

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E3942Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.891	1.993	1.935
	2.095
6ng/ml	1.168	1.032	0.974
	0.896
3ng/ml	0.619	0.567	0.509
	0.515
1.5ng/ml	0.306	0.29	0.232
	0.274
0.75ng/ml	0.095	0.115	0.057
	0.135
0ng/ml	0.062	0.058	0
	0.054

Precision

We measured random samples of E3942Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.6	0.24	4.3
Intra-Assay	2	18	8.9	0.23	2.6
Intra-Assay	3	18	3.6	0.21	5.9

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E3942Hu

$320.00
96T

E3942Hu

$458.00

Add to Cart

Lead time: Within two weeks

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