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Home > Product > ELISA Kit > Human Atp Synthase-Coupling Factor 6, ATP5J ELISA Kit

Human Atp Synthase-Coupling Factor 6, ATP5J ELISA Kit

Human Atp Synthase-Coupling Factor 6, ATP5J ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.88ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.88ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

ATP Synthase-coupling factor 6

Code

E6763Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.88ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

ATP synthase-coupling factor 6, mitochondrial is an enzyme subunit that in humans is encoded by the ATP5PF gene. The F6 subunit is part of the peripheral stalk that links the F1 and FO complexes together, and which acts as a stator to prevent certain subunits from rotating with the central rotary element. The peripheral stalk differs in subunit composition between mitochondrial, chloroplast and bacterial F-ATPases. In mitochondria, the peripheral stalk is composed of one copy each of subunits OSCP (oligomycin sensitivity conferral protein), F6, b and d.[8] There is no homologue of subunit F6 in bacterial or chloroplast F-ATPase, whose peripheral stalks are composed of one copy of the delta subunit (homologous to OSCP), and two copies of subunit b in bacteria, or one copy each of subunits b and b' in chloroplasts and photosynthetic bacteria.

UniProt accession

MASS(Da)

12,588

GeneID

522

Synonyms

ATP Synthase peripheral stalk subunit F6;ATP Synthase-coupling factor 6, mitochondrial;ATP5;ATP5A;ATP5J;ATPase subunit F6;ATPM;CF6;F6;

Gene names

ATP5PF

Research area

Tags & Cell Markers; Signal Transduction; Metabolism

Target protein

ATP5J

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E6763Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.349 2.342 2.292
2.336
400ng/L 1.266 1.255 1.205
1.244
200ng/L 0.688 0.681 0.631
0.674
100ng/L 0.468 0.485 0.435
0.503
50ng/L 0.178 0.208 0.158
0.238
0ng/L 0.048 0.05 0
0.052

Precision

We measured random samples of E6763Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 85.65 5.42 6.33
Intra-Assay 2 18 236.74 12.52 5.29
Intra-Assay 3 18 512.18 12.83 2.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E6763Hu

    $320.00

  • 96T

    E6763Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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