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Home > Product > ELISA Kit > Human E3 Ubiquitin-protein Ligase Dtx3L, DTX3L ELISA Kit

Human E3 Ubiquitin-protein Ligase Dtx3L, DTX3L ELISA Kit

Human E3 Ubiquitin-protein Ligase Dtx3L, DTX3L ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.01ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.01ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

E3 ubiquitin-protein ligase DTX3L

Code

E7314Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.01ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses (PubMed:12670957, PubMed:19818714, PubMed:26479788, PubMed:23230272). Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4 (PubMed:28525742). In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1) (PubMed:19818714). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me) (PubMed:19818714). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication (PubMed:26479788). Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (PubMed:24790097). In addition, required for the recruitment of HGS and STAM to early endosomes (PubMed:24790097). In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteosomal-mediated degradation (PubMed:26479788).

UniProt accession

MASS(Da)

83,554

GeneID

151636

Synonyms

BBAP;B-lymphoma- and BAL-associated protein;DTX3L;E3 ubiquitin-protein ligase DTX3L;Protein deltex-3-like;Rhysin2;Rhysin-2;RING-type E3 ubiquitin transferase DTX3L;RNF143;

Gene names

DTX3L

Research area

Epigenetics and Nuclear Signaling

Target protein

DTX3L

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E7314Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.791 1.73 1.694
1.669
800ng/L 1.064 1.082 1.047
1.101
400ng/L 0.474 0.494 0.458
0.514
200ng/L 0.204 0.238 0.203
0.272
100ng/L 0.126 0.12 0.084
0.114
0ng/L 0.028 0.036 0
0.044

Precision

We measured random samples of E7314Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1324.13 52.42 3.96
Intra-Assay 2 18 721.58 36.69 5.08
Intra-Assay 3 18 348.89 16.64 4.77

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E7314Hu

    $320.00

  • 96T

    E7314Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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