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Home > Product > ELISA Kit > Human Bcl2 Antagonist of Cell Death, BAD ELISA Kit

Human Bcl2 Antagonist of Cell Death, BAD ELISA Kit

Human Bcl2 Antagonist of Cell Death, BAD ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.42ng/L

Detection range: 5-1800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.42ng/L

Detection range: 5-1800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Bcl2 Antagonist of Cell Death

Code

E4102hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.42ng/L

Detection range

5-1800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

UniProt accession

MASS(Da)

18,392

GeneID

572

Synonyms

BAD;Bcl2 antagonist of cell death;Bcl2-associated agonist of cell death;Bcl-2-binding component 6;Bcl2-L-8;Bcl-2-like protein 8;Bcl-xL/Bcl-2-associated death promoter

Gene names

BAD

Research area

Cancer

Target protein

BAD

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Bcl2 Antagonist of Cell Death, BAD ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1000ng/L 1.995 1.875 1.826
1.755
500ng/L 0.951 0.918 0.869
0.885
250ng/L 0.548 0.49 0.441
0.432
125ng/L 0.24 0.245 0.196
0.25
62.5ng/L 0.067 0.105 0.057
0.144
0ng/L 0.052 0.049 0
0.046

Precision

We measured random samples of E4102hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 602.8 25.41 4.2
Intra-Assay 2 18 795.6 22.8 2.9
Intra-Assay 3 18 248.9 11.95 4.8

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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