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Home > Product > ELISA Kit > Human Cyclin Dependent Kinase 9, CDK9 ELISA Kit

Human Cyclin Dependent Kinase 9, CDK9 ELISA Kit

Human Cyclin Dependent Kinase 9, CDK9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.56ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.56ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cyclin Dependent Kinase 9

Code

E4386hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.56ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein kinase involved in the regulation of transcription (PubMed: 10574912, PubMed: 10757782, PubMed: 11145967, PubMed: 11575923, PubMed: 11809800, PubMed: 11884399, PubMed: 14701750, PubMed: 16109376, PubMed: 16109377, PubMed: 20930849, PubMed: 28426094). Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP (PubMed: 10574912, PubMed: 10757782, PubMed: 11145967, PubMed: 11575923, PubMed: 11809800, PubMed: 11884399, PubMed: 14701750, PubMed: 16109376, PubMed: 16109377, PubMed: 20930849, PubMed: 28426094). This complex is inactive when in the 7SK snRNP complex form (PubMed: 10574912, PubMed: 10757782, PubMed: 11145967, PubMed: 11575923, PubMed: 11809800, PubMed: 11884399, PubMed: 14701750, PubMed: 16109376, PubMed: 16109377, PubMed: 20930849, PubMed: 28426094). Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELF (PubMed: 9857195, PubMed: 10912001, PubMed: 11112772, PubMed: 12037670, PubMed: 20081228, PubMed: 20980437, PubMed: 21127351). Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling) (PubMed: 17956865, PubMed: 18362169). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis (PubMed: 10393184, PubMed: 11112772). P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export (PubMed: 15564463, PubMed: 19575011, PubMed: 19844166). Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing (PubMed: 15564463, PubMed: 19575011, PubMed: 19844166). The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro (PubMed: 21127351). Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage (PubMed: 20493174). In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6 (PubMed: 20493174). Promotes cardiac myocyte enlargement (PubMed: 20081228). RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription (PubMed: 21127351). AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect (PubMed: 9857195, PubMed: 10912001, PubMed: 11112772). The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (PubMed: 12037670).

UniProt accession

MASS(Da)

42,778

GeneID

1025

Synonyms

C-2K;CDK 9;CDK9;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Cyclin-dependent kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit

Gene names

CDK9

Research area

Cell Biology; Microbiology; Epigenetics and Nuclear Signaling; Immunology

Target protein

CDK9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Cyclin Dependent Kinase 9, CDK9 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 1.925 1.973 1.923
2.022
800ng/L 0.954 0.964 0.913
0.973
400ng/L 0.47 0.482 0.432
0.494
200ng/L 0.248 0.23 0.18
0.212
100ng/L 0.142 0.154 0.103
0.165
0ng/L 0.064 0.05 0
0.036

Precision

We measured random samples of E4386hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 631.3 27.71 4.4
Intra-Assay 2 18 1291.1 32.47 2.5
Intra-Assay 3 18 487.3 23.38 4.8

Protocols

References

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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