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Home > Product > ELISA Kit > Human Hla Class 2 Histocompatibility Antigen Gamma Chain, CD74 ELISA Kit

Human Hla Class 2 Histocompatibility Antigen Gamma Chain, CD74 ELISA Kit

Human Hla Class 2 Histocompatibility Antigen Gamma Chain, CD74 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Hla Class 2 Histocompatibility Antigen Gamma Chain

Code

E4489Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.27ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays a critical role in MHC class II antigen processing by stabilizing Peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic Peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. [Class-II-associated invariant chain Peptide]: Binds to the Peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic Peptides to the MHC class II complex until its release by HLA-DM in the endosome. [Isoform p41]: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed: 32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed: 32855215).

UniProt accession

MASS(Da)

33,516

GeneID

972

Synonyms

CD 74;CD antigen CD74;CD74;Class-II-associated invariant chain Peptide;CLIP;HLA class II histocompatibility antigen gamma chain;HLA-DR antigens-associated invariant chain;Ia antigen-associated invariant chain;Ii;

Gene names

CD74

Research area

Immunology

Target protein

CD74

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4489Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 1.876 1.85 1.798
1.824
32ng/ml 1.134 1.037 0.986
0.941
16ng/ml 0.594 0.557 0.506
0.521
8ng/ml 0.2 0.2 0.148
0.2
4ng/ml 0.085 0.083 0.031
0.08
0ng/ml 0.059 0.052 0
0.044

Precision

We measured random samples of E4489Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 34.3 1.69 4.9
Intra-Assay 2 18 47.3 1.48 3.1
Intra-Assay 3 18 12 0.44 3.7

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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